Goat anti-Wilms tumor 1 / WT1 Antibody

Pep ELISA, WB

Goat anti-Wilms tumor 1 / WT1 Antibody
Principle Name: Wilms tumor 1 / WT1
Official Symbol: WT1
All Name and Symbols: WT1; Wilms tumor 1; GUD; WAGR; WIT-2; WT33
Accession Number(s): NP_000369.3; NP_077742.2; NP_077743.2; NP_077744.3
Peptide Sequence: QDPASTCVPEPASQH, is from N Terminus
Human Gene ID(s): 7490
Important Comments: This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 55kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3kDa according to NP_000369.3). Recommended concentration: 1-3ug/ml.