Goat anti-Serotonin receptor 3A / HTR3A Antibody

Goat anti-Serotonin receptor 3A / HTR3A Antibody
Principle Name: Serotonin receptor 3A / HTR3A
Official Symbol: HTR3A
All Name and Symbols: HTR3A; serotonin receptor; 5-hydroxytryptamine (serotonin) receptor 3A; 5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3; 5-hydroxytryptamine (serotonin) receptor-3; 5HT3 serotonin receptor; Serotonin-gated ion channel receptor; truncated receptor, containing only 3 t
Accession Number(s): NP_998786.2; NP_000860.2; NP_001155244.1
Peptide Sequence: GPQDFEKSPRDR, is from internal region
Human Gene ID(s): 3359
Important Comments: This antibody is expected to recognise all three reported isoforms (NP_998786.2; NP_000860.2; NP_001155244.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC, ICC, IFPeptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.1. Recommended concentration: 0.3-1ug/ml.
An additional band of unknown identity was also consistently observed at 200kDa. This band was suc
Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml.
Reference(s): Dremencov E, Weizmann Y, Kinor N, Gispan-Herman I, Yadid G. Modulation of dopamine transmission by 5HT2C and 5HT3 receptors: a role in the antidepressant response. Curr Drug Targets. 2006 Feb; 7(2):165-75. Review. .PMID: 16475958