| Description |
Goat anti-Munc13-4 / UNC13D (Internal) Antibody
Principle Name: Munc13-4 / UNC13D (Internal)
Official Symbol: UNC13D
All Name and Symbols: UNC13D; Munc13-4; unc-13 homolog D (C. elegans) ; FHL3; HLH3; HPLH3; unc-13 homolog D
Accession Number(s): NP_954712.1
Peptide Sequence: ETQKHKKDLHPLFD, is from internal region
Human Gene ID(s): 201294
Non-Human GeneID(s): 70450 (mouse); 192177 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human, Mouse
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration, 2.5ug/ml.
In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data
Reference(s): Zur Stadt U, Beutel K, Kolberg S, Schneppenheim R, Kabisch H, Janka G, Hennies HC. Mutation spectrum in children with primary hemophagocytic lymphohistiocytosis: molecular and functional analyses of PRF1, UNC13D, STX11, and RAB27A. Hum Mutat. 2006 Jan; 27(1):62-8. .PMID: 16278825
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