Goat anti-Fibulin 5 / FBLN5 Antibody

Goat anti-Fibulin 5 / FBLN5 Antibody
Principle Name: Fibulin 5 / FBLN5
Official Symbol: FBLN5
All Name and Symbols: FBLN5; fibulin 5 ; ARMD3; DANCE; EVEC; FLJ90059; UP50 ; developmental arteries and neural crest epidermal growth factor-like; urine p50 protein
Accession Number(s): NP_006320.2
Peptide Sequence: RPIKGPREIQLDLE, is from internal region (near the C Terminus)
Human Gene ID(s): 10516
Non-Human GeneID(s): 23876 (mouse); 29158 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 50kDa band observed in Human Colon, Heart and Ovary lysates (calculated MW of 50.2kDa according to NP_006320.2). Recommended concentration: 0.1-0.3ug/ml.
An additional band of unknown identity was also consistently observed at 23kDa
Reference(s): Albig AR, Neil JR, Schiemann WP. Fibulins 3 and 5 antagonize tumor angiogenesis in vivo. Cancer Res. 2006 Mar 1; 66(5):2621-9. .PMID: 16510581