Goat anti-Lipin 1 (Internal) Antibody

Goat anti-Lipin 1 (Internal) Antibody
Principle Name: Lipin 1 (Internal)
Official Symbol: LPIN1
All Name and Symbols: LPIN1; lipin 1 ; DKFZp781P1796; KIAA0188
Accession Number(s): NP_663731.1
Peptide Sequence: NKTDSPSRKRDK, is from internal region
Human Gene ID(s): 23175
Non-Human GeneID(s): 14245 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave bands at approx 50kDa and 26kDa in Human Adipose lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated
Reference(s): Suviolahti E, Reue K, Cantor RM, Phan J, Gentile M, Naukkarinen J, Soro-Paavonen A, Oksanen L, Kaprio J, Rissanen A, Salomaa V, Kontula K, Taskinen MR, Pajukanta P, Peltonen L. Cross-species analyses implicate Lipin 1 involvement in human glucose metabolism. Hum Mol Genet. 2006 Feb 1; 15(3):377-86. Epub 2005 Dec 15. .PMID: 16357106