| Description |
Goat anti-Syntenin / SDCBP Antibody
Principle Name: Syntenin / SDCBP
Official Symbol: SDCBP
All Name and Symbols: SDCBP; Syntenin; syndecan binding protein (syntenin); MDA-9; ST1; SYCL; TACIP18 ; melanoma differentiation associated protein-9; pro-TGF-alpha cytoplasmic domain-interacting protein 18; scaffold protein Pbp1 syntenin
Accession Number(s): NP_005616.2; NP_001007068.1; NP_001007069; NP_001007070.1; NP_001007071.1
Peptide Sequence: SLEDLKVDKVIQAQ, is from N Terminus
Human Gene ID(s): 6386
Non-Human GeneID(s): 53378 (mouse); 83841 (rat);
Important Comments: This antibody is expected to recognise isoform 1 (NP_005616.2 and NP_001007068.1) and isoform 3 (NP_001007070.1and NP_001007071.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 28-30kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 32.4kDa according to NP_005616.2 and NP_001007068.1 and of 32.3kDa according to NP_001007070.1 and NP_001007071.1). Recommended concentration: 0.01-0.
Reference(s): Latysheva N, Muratov G, Rajesh S, Padgett M, Hotchin NA, Overduin M, Berditchevski F. Syntenin-1 is a new component of tetraspanin-enriched microdomains: mechanisms and consequences of the interaction of syntenin-1 with CD63. Mol Cell Biol. 2006 Aug 14; [Epub ahead of print] .PMID: 16908530
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