Goat anti-ICAM5 precursor Antibody

Goat anti-ICAM5 precursor Antibody
Principle Name: ICAM5 precursor
Official Symbol: ICAM5
All Name and Symbols: ICAM5; intercellular adhesion molecule 5, telencephalin ; TLCN; TLN ; intercellular adhesion molecule 5; telencephalin
Accession Number(s): NP_003250.3
Peptide Sequence: ESPAEGEVFAIQ, is from C Terminus
Human Gene ID(s): 7087
Non-Human GeneID(s): 15898 (mouse); 313785 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave an approx 200-250kDa band in lysates of Human Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature
Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4ug/ml.
Reference(s): Yoshihara Y, Oka S, Nemoto Y, Watanabe Y, Nagata S, Kagamiyama H, Mori K. An ICAM-related neuronal glycoprotein, telencephalin, with brain segment-specific expression. Neuron. 1994 Mar; 12(3):541-53. .PMID: 7794412