| Description |
Goat anti-ICBP90 / UHRF1 Antibody
Principle Name: ICBP90 / UHRF1
Official Symbol: UHRF1
All Name and Symbols: UHRF1; ubiquitin-like, containing PHD and RING finger domains, 1; Np95; ICBP90; FLJ21925; transcription factor ICBP90; RNF106; MGC138707; RING finger protein 106; inverted CCAAT box-binding protein of 90 kDa; nuclear zinc finger protein Np95; transcriptio
Accession Number(s): NP_001041666.1; NP_037414.3
Peptide Sequence: LNQLFPGYGNGR, is from C Terminus
Human Gene ID(s): 29128
Non-Human GeneID(s): 18140 (mouse);
Important Comments: This antibody is expected to recognise both reported isoforms.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 2000.
Western Blot: Approx 100kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 91.1kDa according to NP_037414.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Hopfner, Cancer R
Reference(s): Hopfner R, Mousli M, Jeltsch JM, Voulgaris A, Lutz Y, Marin C, Bellocq JP, Oudet P, Bronner C. ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression. Cancer Res. 2000 Jan 1; 60(1):121-8..PMID: 10646863
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