Goat anti-CNTFR Antibody

Pep ELISA, WB

Goat anti-CNTFR Antibody
Principle Name: CNTFR
Official Symbol: CNTFR
All Name and Symbols: CNTFR; ciliary neurotrophic factor receptor ; MGC1774 ; CNTFR alpha; ciliary neurotrophic factor receptor alpha precursor
Accession Number(s): NP_671693.1; NP_001833.1
Peptide Sequence: CRSNTYPKGFY, is from internal region
Human Gene ID(s): 1271
Non-Human GeneID(s): 12804 (mouse); 313173 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_671693.1; NP_001833.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 38-40kDa band observed in mouse brain lysates (calculated MW of 40.8kDa according to mouse NP_057882.1). Recommended concentration: 0.3-1ug/ml.
Reference(s): Yokota H, Yoshikawa M, Hirabayashi H, Nakase H, Uranishi R, Nishimura F, Sugie Y, Ishizaka S, Sakaki T. Expression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha) following experimental intracerebral hemorrhage in rats. Neurosci Lett. 2005 Apr 4; 377(3):170-5. Epub 2005 Jan 4. .PMID: 15755520