Goat anti-ARF1 (variants 1-4) Antibody

Goat anti-ARF1 (variants 1-4) Antibody
Principle Name: ARF1 (variants 1-4)
Official Symbol: ARF1
All Name and Symbols: #Deleted
Accession Number(s): NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1
Peptide Sequence: EGLDWLSNQLRNQK, is from C Terminus
Human Gene ID(s): 375; 376, 377, 378
Non-Human GeneID(s): 11840 (mouse); 64310 (rat);
Important Comments: This antibody is expected to recognize all four reported isoforms.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 18kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kDa according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). Recommended concentration: 1-3ug/ml.
Reference(s): Dubois T, Paleotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P. Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics. Nat Cell Biol. 2005 Apr; 7(4):353-64. Epub 2005 Mar 27. .PMID: 15793564