Goat anti-Semaphorin 3E (internal) Antibody

Pep ELISA, IHC

Goat anti-Semaphorin 3E (internal) Antibody
Principle Name: Semaphorin 3E (internal)
Official Symbol: SEMA3E
All Name and Symbols: SEMA3E; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E; KIAA0331; M-SEMAH; M-SemaK; M-sema H; SEMAH; coll-5 ; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E; semaphorin 3E
Accession Number(s): NP_036563.1; NP_001171600.1
Peptide Sequence: NGGRYGTTKDYPDD, is from internal region
Human Gene ID(s): 9723
Non-Human GeneID(s): 20349 (mouse);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_036563.1; NP_001171600.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments gave an approx 35kDa band in Human Prostate lysates after 0.03ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 8
Immunohistochemistry: In paraffin embedded Human Prostate shows membranous staining of secretory cells in the gland. Recommended concentration, 4-6ug/ml.
Reference(s): Gu C, Yoshida Y, Livet J, Reimert DV, Mann F, Merte J, Henderson CE, Jessell TM, Kolodkin AL, Ginty DD. Semaphorin 3E and plexin-D1 control vascular pattern independently of neuropilins. Science. 2005 Jan 14; 307(5707):265-8. Epub 2004 Nov 18. .PMID: 15550623