| Description |
Goat anti-TCF1 / HNF1 Antibody
Principle Name: TCF1 / HNF1
Official Symbol: HNF1A
All Name and Symbols: TCF1; transcription factor 1, hepatic LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor ; HNF1; HNF1A; LFB1; MODY3 ; Interferon production regulator factor (HNF1); albumin proximal factor; hepatic nuclear factor 1; maturity onset diabetes of t
Accession Number(s): NP_000536.3
Peptide Sequence: DELPTKKGRRNRFK, is from internal region
Human Gene ID(s): 6927
Non-Human GeneID(s): 21405 (mouse); 24817 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Preliminary experiments in human liver and kidney lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Reference(s): Hansen SK, Parrizas M, Jensen ML, Pruhova S, Ek J, Boj SF, Johansen A, Maestro MA, Rivera F, Eiberg H, Andel M, Lebl J, Pedersen O, Ferrer J, Hansen T. Genetic evidence that HNF-1alpha-dependent transcriptional control of HNF-4alpha is essential for human pancreatic beta cell function. J Clin Invest. 2002 Sep; 110(6):827-33. .PMID: 12235114
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