Goat anti-RNF213 / C17orf27 (internal) Antibody

Pep ELISA, IHC

Goat anti-RNF213 / C17orf27 (internal) Antibody
Principle Name: RNF213 / C17orf27 (internal)
Official Symbol: RNF213
All Name and Symbols: ring finger protein 213; RNF213; C17orf27; chromosome 17 open reading frame 27; KIAA1554
Accession Number(s): NP_065965.3
Peptide Sequence: KNPQTQTEERFRPQ, is from internal region
Human Gene ID(s): 57674
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration.
Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 1.25ug/ml.
Reference(s): Zody MC et al DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage. Nature. 2006 Apr 20; 440(7087):1045-9.PMID: 16625196