Goat anti-AGTR1 / AT1 Antibody

Pep ELISA, WB

Goat anti-AGTR1 / AT1 Antibody
Principle Name: AGTR1 / AT1
Official Symbol: AGTR1
All Name and Symbols: AGTR1; AT1; angiotensin II receptor, type 1 ; AG2S; AGTR1A; AGTR1B; AT1B; AT2R1; AT2R1A; AT2R1B; HAT1R; angiotensin receptor 1; angiotensin receptor 1B; type-1B angiotensin II receptor
Accession Number(s): NP_000676.1; NP_004826.2; NP_033611.1; NP_114038.1; NP_114438.1
Peptide Sequence: EIQKNKPRNDDIFK, is from internal region
Human Gene ID(s): 185
Non-Human GeneID(s): 11607 (mouse); 24180 (rat);
Important Comments: All reported variants represent identical protein (NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1 and NP_114438.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 38kDa band observed in Liver lysates of Human and Mouse (calculated MW of 41.1kDa according to NP_000676.1, NP_004826.2, NP_033611.1, NP_114038.1 and NP_114438.1). Recommended concentration: 0.3-1ug/ml.
Reference(s): Wang M, Jiang C, Wang C, Yao Q. The Role of brain angiotensinergic AT(1) receptor in the carbachol-induced natriuresis and expression of nNOS in the Locus Coeruleus (LC) and proximal convoluted tubule (PCT). Physiol Res. 2006 Aug 22; [Epub ahead of print] .PMID: 16925472