Goat anti-TCF3 / ITF1 Antibody

Pep ELISA, WB, IHC

Goat anti-TCF3 / ITF1 Antibody
Principle Name: TCF3 / ITF1
Official Symbol: TCF3
All Name and Symbols: TCF3; ITF1; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); E2A; MGC129647; MGC129648 ; E2A immunoglobulin enhancer-binding factor E12/E47; immunoglobulin transcription factor 1; kappa-E2-binding factor; transcription factor
Accession Number(s): NP_003191.1
Peptide Sequence: KAPRARTSPDEDED, is from internal region
Human Gene ID(s): 6929
Important Comments: This antibody is expected to recognize reported isoform E12 (NP_003191.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5ug/ml.
Reference(s): Greenbaum S, Lazorchak AS, Zhuang Y. Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes. J Biol Chem. 2004 Oct 22; 279(43):45028-35.PMID: 15310760