Goat anti-ACYP1 (isoform a) Antibody

Goat anti-ACYP1 (isoform a) Antibody
Principle Name: ACYP1 (isoform a)
Official Symbol: ACYP1
All Name and Symbols: ACYP1; acylphosphatase 1, erythrocyte (common) type ; ACYPE ; erythrocyte acylphosphatase 1
Accession Number(s): NP_001098.1
Peptide Sequence: PKSHIDKANFNNEK, is from internal region
Human Gene ID(s): 97
Non-Human GeneID(s): 66204 (mouse);
Important Comments: This antibody is expected to recognize isoform a (NP_001098.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 15kDa band observed in Human Testis lysates (calculated MW of 11.3kDa according to NP_001098.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
Reference(s): Paoli P, Pazzagli L, Giannoni E, Caselli A, Manao G, Camici G, Ramponi G. A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase. J Biol Chem. 2003 Jan 3; 278(1):194-9. Epub 2002 Oct 29. .PMID: 12409302