Goat anti-P4HA1 Antibody

Goat anti-P4HA1 Antibody
Principle Name: P4HA1
Official Symbol: P4HA1
All Name and Symbols: P4HA1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; 4-PH alpha-1; P4HA ; prolyl 4-hydroxylase, alpha I subunit
Accession Number(s): NP_000908.2; NP_001017962.1
Peptide Sequence: DFARKDEPDAFKE, is from internal region
Human Gene ID(s): 5033
Non-Human GeneID(s): 18451 (mouse); 64475 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 60kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1). Recommended concentration: 0.1-0.1ug/ml.
An additional band of unknown identity was also consistent
Immunohistochemistry: Paraffin embedded Human Skin and Spleen. Recommended concentration: 5ug/ml.
Reference(s): Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI. Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer. J Biol Chem. 1997 Jul 11; 272(28):17342-8. .PMID: 9211872