Goat anti-PTPN6 / SHP1 (internal region) Antibody

Goat anti-PTPN6 / SHP1 (internal region) Antibody
Principle Name: PTPN6 / SHP1 (internal region)
Official Symbol: PTPN6
All Name and Symbols: PTPN6; SHP1; protein tyrosine phosphatase, non-receptor type 6 ; HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1; SHP-1L; 70 kda SHP-1L protein; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; protein-tyrosine phosphatase 1C
Accession Number(s): NP_536858.1; NP_002822.2
Peptide Sequence: KASRTSSKHKEE, is from internal region
Human Gene ID(s): 5777
Non-Human GeneID(s): 15170 (mouse); 116689 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_536858.1and NP_002822.2).
Please note this antibody was designed using the mouse sequence, which differs by 1 amino acid from the human sequence.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human, Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 65kDa band observed in Human Bone Marrow and Liver lysates and in Mouse and Rat Spleen and Thymus lysates (calculated MW of 67.6kDa according to NP_536858.1and NP_002822.2). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Dubois MJ, Bergeron S, Kim HJ, Dombrowski L, Perreault M, Fournes B, Faure R, Olivier M, Beauchemin N, Shulman GI, Siminovitch KA, Kim JK, Marette A. The SHP-1 protein tyrosine phosphatase negatively modulates glucose homeostasis. Nat Med. 2006 May; 12(5):549-56. Epub 2006 Apr 16. .PMID: 16617349