Goat anti-PAX8 (internal) Antibody

Goat anti-PAX8 (internal) Antibody
Principle Name: PAX8 (internal)
Official Symbol: PAX8
All Name and Symbols: PAX8; paired box gene 8; HGNC:8622 ; paired domain gene 8; paired box 8
Accession Number(s): NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1
Peptide Sequence: AQPGSDKRKMDD, is from internal region
Human Gene ID(s): 7849
Important Comments: This antibody is expected to recognise all reported isoforms (NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB Trf
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: In transfected HEK293 transiently expressing PAX8A a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.5ug/ml.
Reference(s): Castro P, Rebocho AP, Soares RJ, Magalhaes J, Roque L, Trovisco V, Vieira de Castro I, Cardoso-de-Oliveira M, Fonseca E, Soares P, Sobrinho-Simoes M. PAX8-PPAR{gamma} rearrangement is frequently detected in the follicular variant of papillary thyroid carcinoma. J Clin Endocrinol Metab. 2005 Oct 11; [Epub ahead of print] .PMID: 16219715