Goat anti-Argininosuccinate synthetase 1 Antibody

Goat anti-Argininosuccinate synthetase 1 Antibody
Principle Name: Argininosuccinate synthetase 1
Official Symbol: ASS1
All Name and Symbols: Argininosuccinate synthetase 1; ASS; ASS1; CTLN1; citrulline--aspartate ligase
Accession Number(s): NP_000041.2
Peptide Sequence: ENPKNQAPPGLYTKTQD, is from internal region
Human Gene ID(s): 445
Non-Human GeneID(s): 11898 (mouse); 25698 (rat);
Important Comments: The variants represent identical protein (NP_000041.2 and NP_446464.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF, IP, IHCPeptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 45kDa band observed in Human Kidney lysates (calculated MW of 46.5kDa according to NP_000041.2 and NP_446464.1). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows textured cytoplasm staining in PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.
Reference(s): Hao G, Xie L, Gross SS. Argininosuccinate synthetase is reversibly inactivated by S-nitrosylation in vitro and in vivo. J Biol Chem. 2004 Aug 27; 279(35):36192-200. Epub 2004 Jun 10. .PMID: 15192091