Goat anti-SORBS1/ponsin (internal region) Antibody

Goat anti-SORBS1/ponsin (internal region) Antibody
Principle Name: SORBS1/ponsin (internal region)
Official Symbol: SORBS1
All Name and Symbols: SORBS1; sorbin and SH3 domain containing 1; CAP; DKFZp451C066; DKFZp586P1422; FLAF2; FLJ12406; KIAA1296; R85FL; SH3D5; SH3P12; SORB1 ; Fas-ligand associated factor 2; SH3-domain protein 5; SH3-domain protein 5 (ponsin); c-Cbl associated protein; ponsin
Accession Number(s): NP_006425.2; NP_056200.1; NP_001030126.1; NP_001030127.1; NP_001030128.1; NP_079267.1; NP_001030129.1
Peptide Sequence: SERNDWEPPDKKVD, is from internal region
Human Gene ID(s): 10580
Non-Human GeneID(s): 20411 (mouse);
Important Comments: This antibody is expected to recognise all reported isoforms (NP_006425.2 and NP_056200.1 and NP_001030126.1 and NP_001030127.1 and NP_001030128.1 and NP_079267.1 and NP_001030129.1)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.
Reference(s): Baumann CA, Ribon V, Kanzaki M, Thurmond DC, Mora S, Shigematsu S, Bickel PE, Pessin JE, Saltiel AR. CAP defines a second signalling pathway required for insulin-stimulated glucose transport. Nature. 2000 Sep 14; 407(6801):202-7. .PMID: 11001060