Goat anti-NAT10 / hALP Antibody

Goat anti-NAT10 / hALP Antibody
Principle Name: NAT10 / hALP
Official Symbol: NAT10
All Name and Symbols: NAT10; N-acetyltransferase 10; ALP; DKFZp434C116; FLJ10774; FLJ12179; FLJ23850; KIAA1709; hALP ; N-acetyltransferase-like; N-acetyltransferase-like protein
Accession Number(s): NP_078938.1
Peptide Sequence: KSDKKRKLEAKQ, is from internal region (near the C Terminus)
Human Gene ID(s): 55226
Non-Human GeneID(s): 98956 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Preliminary experiments gave an approx 26kDa band in Human Brain (Cerebellum, Frontal Cortex and Hippocampus) lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band
Immunohistochemistry: In paraffin embedded Human Colon shows nuclear staining in absorptive cells. Recommended concentration, 3-6ug/ml.
Reference(s): Yin J, Ma Z, Selliah N, Shivers DK, Cron RQ, Finkel TH. Effective gene suppression using small interfering RNA in hard-to-transfect human T cells. J Immunol Methods. 2006 May 30; 312(1-2):1-11. Epub 2006 Mar 24. .PMID: 16603179