Goat anti-Gabpb2 (mouse) Antibody

Goat anti-Gabpb2 (mouse) Antibody
Principle Name: Gabpb2 (mouse)
Official Symbol: Gabpb2
All Name and Symbols: Gabpb2; GA binding protein transcription factor, beta 2
Accession Number(s): NP_766100.1; NP_084161.1
Peptide Sequence: KSGRTTEPHTN, is from C Terminus
Human Gene ID(s):
Non-Human GeneID(s): 213054 (mouse); 295263 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms (NP_766100.1; NP_084161.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx 35kDa band in Rat Kidney lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.7kDa
Reference(s): O'Leary DA, Koleski D, Kola I, Hertzog PJ, Ristevski S. Identification and expression analysis of alternative transcripts of the mouse GA-binding protein (Gabp) subunits alpha and beta1. Gene. 2005 Jan 3; 344:79-92. Epub 2004 Nov 23. .PMID: 15656975