Goat anti-SOD1 Antibody

Goat anti-SOD1 Antibody
Principle Name: SOD1
Official Symbol: SOD1
All Name and Symbols: SOD1; superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) ; HGNC:11179; ALS; ALS1; IPOA; SOD ; Cu /Zn superoxide dismutase; Cu/Zn superoxide dismutase; SOD, soluble; indophenoloxidase A; superoxide dismutase (aa 120-154); superoxide
Accession Number(s): NP_000445.1
Peptide Sequence: SRKHGGPKDEERH, is from internal region
Human Gene ID(s): 6647
Non-Human GeneID(s): 20655 (mouse); 24786 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, EIA
Tested Species: Human, Mouse
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 18kDa band observed in Mouse Brain lysates, in purified membranes of cell line HeLa and in lysates of NIH3T3. Approx 20kDa observed in HEK293, HepG2 and MCF7 (calculated MW of 15.9kDa according to human NP_000445.1, mouse NP_035564.1
Reference(s): Ghadge GD, Wang L, Sharma K, Monti AL, Bindokas V, Stevens FJ, Roos RP. Truncated wild-type SOD1 and FALS-linked mutant SOD1 cause neural cell death in the chick embryo spinal cord. Neurobiol Dis. 2006 Jan; 21(1):194-205. Epub 2005 Aug 9.PMID: 16084730