Goat anti-AIF1 / IBA1 (isoforms 1 + 3) Antibody

Goat anti-AIF1 / IBA1 (isoforms 1 + 3) Antibody
Principle Name: AIF1 / IBA1 (isoforms 1 + 3)
Official Symbol: AIF1
All Name and Symbols: AIF1; IBA1; allograft inflammatory factor 1; DASS-82G15.2; AIF-1; IRT-1; Em:AF129756.17; interferon gamma responsive transcript; ionized calcium-binding adapter molecule
Accession Number(s): NP_116573.1; NP_001614.3
Peptide Sequence: YEEKAREKEKP, is from C Terminus
Human Gene ID(s): 199
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 16kDa band observed in Human Lymph Node and Spleen lysates (calculated MW of 16.7kDa according to NP_001614.3. Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4ug/ml.
Reference(s): Kelemen SE, Autieri MV. Expression of allograft inflammatory factor-1 in T lymphocytes: a role in T-lymphocyte activation and proliferative arteriopathies. Am J Pathol. 2005 Aug; 167(2):619-26..PMID: 16049345