Goat anti-PGC1A Antibody

Goat anti-PGC1A Antibody
Principle Name: PGC1A
Official Symbol: PPARGC1A
All Name and Symbols: PGC1A; PPARGC1A; peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; LEM6; PGC-1(alpha); PGC-1v; PGC1; PPARGC1; PPAR gamma coactivator variant form; PPAR gamma coactivator-1; ligand effect modulator-6; peroxisome proliferative activated
Accession Number(s): NP_037393.1
Peptide Sequence: DGLFDDSEDESDK, is from internal region
Human Gene ID(s): 10891
Non-Human GeneID(s): 19017 (mouse); 83516 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 110kDa band observed in Human Skeletal Muscle lysates (calculated MW of 91.0kDa according to NP_037393.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Skel
Reference(s): Handschin C, Kobayashi YM, Chin S, Seale P, Campbell KP, Spiegelman BM. PGC-1alpha regulates the neuromuscular junction program and ameliorates Duchenne muscular dystrophy. Genes Dev. 2007 Apr 1; 21(7):770-83. .PMID: 17403779