Goat anti-Ide (mouse) Antibody

Goat anti-Ide (mouse) Antibody
Principle Name: Ide (mouse)
Official Symbol: Ide
All Name and Symbols: Ide; insulin-degrading enzyme; 1300012G03Rik; 4833415K22Rik; AA675336; AI507533; INSULYSIN; insulinase; insulysin
Accession Number(s): NP_112419.2
Peptide Sequence: QQYNYDRDNIE, is from internal region
Human Gene ID(s):
Non-Human GeneID(s): 15925 (mouse); 25700 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 30kDa. This
Reference(s): Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM. Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease. Neurobiol Aging. 2007 Jun; 28(6):824-30. Epub 2006 Jun 12..PMID: 16769157