Goat anti-TPD52L2 / D54 Antibody

Pep ELISA, WB, IHC

Goat anti-TPD52L2 / D54 Antibody
Principle Name: TPD52L2 / D54
Official Symbol: TPD52L2
All Name and Symbols: TPD52L2; D54; tumor protein D52-like 2 ; DKFZp686A1765; hD54; HCCR-binding protein 2
Accession Number(s): NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1
Peptide Sequence: SGDKPLSDPAP, is from C Terminus
Human Gene ID(s): 7165
Important Comments: This antibody is expected to recognise all reported isoforms (NP_955392.1; NP_955393.1; NP_955394.1; NP_955395.1; NP_003279.2; NP_955391.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 28kDa band observed in Human Breast Cancer and Human Brain lysates (calculated MW of 24.9kDa according to NP_955392.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. See also our D52 and D53 products.
Immunohistochemistry: Paraffin embedded Human Spleen, Tonsil and Brain (Cortex). Recommended concentration: 3.75ug/ml.
Reference(s): Reynolds JL, Mahajan SD, Sykes D, Nair MP. Heroin-Induces Differential Protein Expression by Normal Human Astrocytes (NHA). Am J Infect Dis. 2006; 2(2):49-57.PMID: 17235376