Goat anti-Insulysin / Insulinase Antibody

Goat anti-Insulysin / Insulinase Antibody
Principle Name: Insulysin / Insulinase
Official Symbol: IDE
All Name and Symbols: insulinase; insulysin; insulin-degrading enzyme ; FLJ35968; INSULYSIN
Accession Number(s): NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1
Peptide Sequence: QQYNFDRDNTE, is from internal region
Human Gene ID(s): 3416
Important Comments: This antibody is expected to recognize all reported isoforms (NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). In transfected HEK293 transiently expressing Insulysin a band of approx. 110kDa is observed. This band is not observed in the n
Immunohistochemistry: In paraffin embedded Human Lung shows cytoplasm staining of alveolar macrophages. Recommended concentration, 3-6ug/ml.
Reference(s): Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM. Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease. Neurobiol Aging. 2007 Jun; 28(6):824-30. Epub 2006 Jun 12.PMID: 16769157