Goat anti-Neurofibromin 2 (Zebrafish) Antibody

Pep ELISA, IHC

Goat anti-Neurofibromin 2 (Zebrafish) Antibody
Principle Name: Neurofibromin 2 (Zebrafish)
Official Symbol: nf2
All Name and Symbols: nf2; neurofibromin 2 (bilateral acoustic neuroma) ; MGC123124; NF2a
Accession Number(s): NP_998116.1
Peptide Sequence: EKSKHLQDQLK, is from internal region
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Not yet tested. At this stage we are dependent on researchers in the field for further characterization of this product. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any
Immunohistochemistry: This product has been successfully used by customers on Formalin/PFA-fixed paraffin-embedded sections of Zebrafish Brain Cortex.
Reference(s): Sadler KC, Amsterdam A, Soroka C, Boyer J, Hopkins N. A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease. Development. 2005 Aug; 132(15):3561-72. Epub 2005 Jul 6.PMID: 16000385