Goat anti-FAIM1 Antibody

Pep ELISA, WB, IHC

Goat anti-FAIM1 Antibody
Principle Name: FAIM1
Official Symbol: FAIM
All Name and Symbols: FAIM1; FAIM; Fas apoptotic inhibitory molecule
Accession Number(s): NP_001028202.1; NP_001028203.1; NP_001028204.1; NP_060617.1
Peptide Sequence: KKYMEDRSKTTN, is from internal region
Human Gene ID(s): 55179
Non-Human GeneID(s): 23873 (mouse); 140930 (rat);
Important Comments: This antibody is expected to recognise all reported isoforms (NP_001028202.1; NP_001028203.1; NP_001028204.1; NP_060617.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 25kDa band observed in Human Lymph Node lysates (calculated MW of 24.0kDa according to NP_001028202.1). Recommended concentration: 0.5-1ug/ml.
Immunohistochemistry: In paraffin embedded Human Placenta shows cytoplasm staining of trophoblasts. Recommended concentration, 2-3ug/ml.
Reference(s): Schneider TJ, Fischer GM, Donohoe TJ, Colarusso TP, Rothstein TL. A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes. J Exp Med. 1999 Mar 15; 189(6):949-56. .PMID: 10075978