Goat anti-DNMT1 Antibody

Pep ELISA, WB, IHC

Goat anti-DNMT1 Antibody
Principle Name: DNMT1
Official Symbol: DNMT1
All Name and Symbols: DNMT1; DNA (cytosine-5-)-methyltransferase 1; CXXC9; DNMT; FLJ16293; MCMT; MGC104992; DNA methyltransferase 1
Accession Number(s): NP_001370.1
Peptide Sequence: RFESPPKTQPTEDN, is from internal egion
Human Gene ID(s): 1786
Non-Human GeneID(s): 13433 (mouse); 84350 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 170kDa band observed in lysates of cell line Jurkat (calculated MW of 183kDa according to NP_001370.1). Recommended concentration: 0.5-1.5ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in PCT. Recommended concentration, 3-6ug/ml.
Reference(s): Dion V, Lin Y, Hubert L Jr, Waterland RA, Wilson JH. Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline. Hum Mol Genet. 2008 May 1; 17(9):1306-17. Epub 2008 Feb 5.PMID: 18252747