Goat anti-OX1R and OX2R Antibody

Goat anti-OX1R and OX2R Antibody
Principle Name: OX1R and OX2R
Official Symbol: HCRTR1
All Name and Symbols: OX1R; OX2R; HCRTR1; hypocretin (orexin) receptor 1; hypocretin receptor 1; hypocretin receptor-1; orexin receptor 1; orexin receptor-1; HCRTR2; hypocretin (orexin) receptor 2; hypocretin receptor 2; hypocretin receptor-2; orexin receptor 2; orexin recepto
Accession Number(s): NP_001516.2; NP_001517.2
Peptide Sequence: YNFLSGKFREQFK, is from internal region
Human Gene ID(s): 3061; 3062
Non-Human GeneID(s): 230777 (mouse); 25593 (rat);
Important Comments: This antibody is expected to recognise both the human proteins OX1R and OX2R.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WBPeptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 48kDa band observed in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates (calculated MW of 47.5kDa according to NP_001516.1 and NP_001517.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Reference(s): Fukunaka Y, Shinkai T, Hwang R, Hori H, Utsunomiya K, Sakata S, Naoe Y, Shimizu K, Matsumoto C, Ohmori O, Nakamura J The orexin 1 receptor (HCRTR1) gene as a susceptibility gene contributing to polydipsia-hyponatremia in schizophrenia Neuromolecular Med. 2007; 9(4):292-7.PMID: 17999203