Goat anti-GPR120 Antibody

Pep ELISA, IHC

Goat anti-GPR120 Antibody
Principle Name: GPR120
Official Symbol: GPR120
All Name and Symbols: GPR120; G protein-coupled receptor 120; GPR129; GT01; MGC119984; PGR4; G protein-coupled receptor 129; G protein-coupled receptor PGR4; G-protein-coupled receptor GT01
Accession Number(s): NP_859529.2
Peptide Sequence: YSESHQIRVSQQD, is from internal region
Human Gene ID(s): 338557
Non-Human GeneID(s): 107221 (mouse); 294075 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments in Human Colon and Duodenum lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry: In paraffin embedded Human Colon shows specific staining of Lymphoid Aggregates. Recommended concentration: 2-4ug/ml.
Reference(s): Gotoh C, Hong YH, Iga T, Hishikawa D, Suzuki Y, Song SH, Choi KC, Adachi T, Hirasawa A, Tsujimoto G, Sasaki S, Roh SG. The regulation of adipogenesis through GPR120. Biochem Biophys Res Commun. 2007 Mar 9; 354(2):591-7. Epub 2007 Jan 16.PMID: 17250804