Goat anti-APOBEC3G Antibody

Pep ELISA, WB, IHC

Goat anti-APOBEC3G Antibody
Principle Name: APOBEC3G
Official Symbol: APOBEC3G
All Name and Symbols: APOBEC3G; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G; ARP9; CEM15; FLJ12740; MDS019; bK150C2.7; dJ494G10.1 ; DNA dC
dU editing enzyme; OTTHUMP00000028911; apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G; phorbo
Accession Number(s): NP_068594.1
Peptide Sequence: DEHSQDLSGRLR, is from C Terminus
Human Gene ID(s): 60489
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx. 45kDa band observed in lysates of cell line Daudi (calculated MW of 46.4kDa according to NP_068594.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Testis and Kidney. Recommended concentration: 2.5ug/ml.
Reference(s): Derse D, Hill SA, Princler G, Lloyd P, Heidecker G. Resistance of human T cell leukemia virus type 1 to APOBEC3G restriction is mediated by elements in nucleocapsid. Proc Natl Acad Sci U S A. 2007 Feb 13; [Epub ahead of print].PMID: 17299050