Goat anti-SEPT2 Antibody

Pep ELISA, WB, IHC

Goat anti-SEPT2 Antibody
Principle Name: SEPT2
Official Symbol: SEPT2
All Name and Symbols: SEPT2; septin 2; DIFF6; KIAA0158; NEDD5; Pnutl3; hNedd5; neural precursor cell expressed, developmentally down-regulated 5
Accession Number(s): NP_001008491.1
Peptide Sequence: SKQQPTQFINPET, is from N terminus
Human Gene ID(s): 4735
Non-Human GeneID(s): 18000 (mouse); 117515 (rat);
Important Comments: Reported variants represent identical protein: NP_001008492.1, NP_006146.1, NP_004395.1, NP_001008491.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx. 40kDa band observed in Human Testis lysates (calculated MW of 41.5kDa according to NP_001008491.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Breast, Tonsil and Brain (Cortex). Recommended concentration: 2.5ug/ml.
Reference(s): Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclear
accumulation of NCK mediated by SOCS7. Cell. 2007 Sep 7; 130(5):837-50.PMID: 17803907