Goat anti-PHOX2B Antibody

Goat anti-PHOX2B Antibody
Principle Name: PHOX2B
Official Symbol: PHOX2B
All Name and Symbols: PHOX2B; paired-like homeobox 2b; NBPhox; PMX2B; PHOX2B homeodomain protein; neuroblastoma paired-type homeobox protein; paired mesoderm homeobox 2b
Accession Number(s): NP_003915.2
Peptide Sequence: SRDDESKEAKSTDP, is from internal region
Human Gene ID(s): 8929
Non-Human GeneID(s): 18935 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Preliminary experiments in Human Brain (Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with
Reference(s): McGaughey DM, Vinton RM, Huynh J, Al-Saif A, Beer MA, McCallion AS. Metrics of sequence constraint overlook regulatory sequences in an exhaustive analysis at phox2b. Genome Res. 2008 Feb; 18(2):252-60. Epub 2007 Dec 10.PMID: 18071029