Goat anti-S100A9 Antibody

Pep ELISA, WB, IHC

Goat anti-S100A9 Antibody
Principle Name: S100A9
Official Symbol: S100A9
All Name and Symbols: S100A9; S100 calcium binding protein A9; 60B8AG; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); calgranulin B
Accession Number(s): NP_002956.1
Peptide Sequence: DTNADKQLSFEEF , is from internal region
Human Gene ID(s): 6280
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 16kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 13.2kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings (ca.15kDa) in literature with different antibodie
Immunohistochemistry: In paraffin embedded Human Lung shows nuclear staining in select cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.
Reference(s): Corbin BD, Seeley EH, Raab A, Feldmann J, Miller MR, Torres VJ, Anderson KL, Dattilo BM, Dunman PM, Gerads R, Caprioli RM, Nacken W, Chazin WJ, Skaar EP. Metal chelation and inhibition of bacterial growth in tissue abscesses. Science. 2008 Feb 15; 319(5865):962-5.PMID: 18276893