Goat anti-NY-ESO-1 Antibody

Goat anti-NY-ESO-1 Antibody
Principle Name: NY-ESO-1
Official Symbol: CTAG1B
All Name and Symbols: CTAG1B; cancer/testis antigen 1B; CTAG; CTAG1; ESO1; LAGE-2; LAGE2B; NY-ESO-1; New York esophageal squamous cell carcinoma 1; cancer antigen 3
Accession Number(s): NP_001318.1
Peptide Sequence: AELARRSLAQDAP, is from internal region
Human Gene ID(s): 1485
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, MA
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments gave an approx. 28kDa band in Human Testis lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18
Reference(s): Adams S, O'Neill DW, Nonaka D, Hardin E, Chiriboga L, Siu K, Cruz CM, Angiulli A, Angiulli F, Ritter E, Holman RM, Shapiro RL, Berman RS, Berner N, Shao Y, Manches O, Pan L, Venhaus RR, Hoffman EW, Jungbluth A, Gnjatic S, Old L, Pavlick AC, Bhardwaj N. Immunization of Malignant Melanoma Patients with Full-Length NY-ESO-1 Protein Using TLR7 Agonist Imiquimod as Vaccine Adjuvant. J Immunol. 2008 Jul 1; 181(1):776-84.PMID: 18566444