Goat anti-Netrin 1 Antibody

Pep ELISA, WB, IHC

Goat anti-Netrin 1 Antibody
Principle Name: Netrin 1
Official Symbol: NTN1
All Name and Symbols: netrin 1; HGNC:8029; NTN1; NTN1L; Netrin-1; netrin 1, mouse, homolog of
Accession Number(s): NP_004813.2
Peptide Sequence: KFQQREKKGKCKKA, is from C Terminus
Human Gene ID(s): 9423
Non-Human GeneID(s): 18208 (mouse); 114523 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx. 70kDa band observed in Rat Brain lysates (calculated MW of 67.7kDa according to Human NP_004813.1 and 67.8kDa according to Rat NP_446183.1). Recommended concentration: 0.5-1.5ug/ml.
Immunohistochemistry: Paraffin embedded Human Intestine. Recommended concentration: 3.75ug/ml.
Reference(s): Lin L, Rao Y, Isacson O. Netrin-1 and slit-2 regulate and direct neurite growth of ventral midbrain dopaminergic neurons. Mol Cell Neurosci. 2005 Mar; 28(3):547-55. .PMID: 15737744