Goat anti-FAS / CD95 Antibody

Pep ELISA, WB, IHC

Goat anti-FAS / CD95 Antibody
Principle Name: FAS / CD95
Official Symbol: FAS
All Name and Symbols: FAS; Fas (TNF receptor superfamily, member 6); ALPS1A; APO-1; APT1; CD95; FAS1; FASTM; TNFRSF6; APO-1 cell surface antigen; CD95 antigen; Fas AMA; Fas antigen; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; apoptosis antig
Accession Number(s): NP_000034.1; NP_690610.1; NP_690611.1
Peptide Sequence: KTCRKHRKENQGSH, is from internal region
Human Gene ID(s): 355
Important Comments: This antibody is expected to recognize reported isoforms 1 (NP_000034.1), 2 (NP_690610.1) and 3 (NP_690611.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 37kDa band observed in lysates of cell line MOLT4 (calculated MW of 37.7kDa according to NP_000034.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75ug/ml.
Reference(s): Blomberg J, Ruuth K, Jacobsson M, Höglund A, Nilsson JA, Lundgren E, Reduced FAS transcription in clones of U937 cells that have acquired resistance to Fas-induced apoptosis. FEBS J. 2009 Jan 276 (2): 497-508.PMID: 19076218