Goat anti-IKAROS / IKZF1 Antibody

Pep ELISA, WB, IHC

Goat anti-IKAROS / IKZF1 Antibody
Principle Name: IKAROS / IKZF1
Official Symbol: IKZF1
All Name and Symbols: IKZF1; IKAROS family zinc finger 1 (Ikaros); Hs.54452; IK1; IKAROS; LYF1; PRO0758; ZNFN1A1; hIk-1; CLL-associated antigen KW-6; Ikaros (zinc finger protein); zinc finger protein, subfamily 1A, 1 (Ikaros); lymphoid transcription factor LyF-1
Accession Number(s): NP_006051.1
Peptide Sequence: DTPYDSSASYEKEN, is from internal region
Human Gene ID(s): 10320
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 1000.
Western Blot: Approx 65kDa band observed in lysates of cell line MOLT4 (calculated MW of 57.5kDa according to NP_006051.1). Recommended concentration: 1-3ug/ml.
Similar results were obtained with EB07847, which was developed against a different part of t
Immunohistochemistry: In paraffin embedded Human Tonsil shows textured staining in some nuclei of the lymphoid tissue. Recommended concentration, 2.5-3.8ug/ml.
Reference(s): Loeper S, Asa SL, Ezzat S, Ikaros modulates cholesterol uptake: a link between tumor suppression and differentiation. Cancer research 2008 May 68 (10): 3715-23.PMID: 18483254