Goat anti-TRIM29 / ATDC Antibody

Goat anti-TRIM29 / ATDC Antibody
Principle Name: TRIM29 / ATDC
Official Symbol: TRIM29
All Name and Symbols: TRIM29; tripartite motif-containing 29; ATDC; FLJ36085; ataxia-telangiectasia group D-associated protein; tripartite motif protein TRIM29
Accession Number(s): NP_036233.2; NP_001317311.1
Peptide Sequence: EKDRIKSFTTNE, is from internal region
Human Gene ID(s): 23650
Non-Human GeneID(s): 72169 (mouse); 300656 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF, FC, IHC
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8
Immunohistochemistry: In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration: 3.75ug/ml.
Reference(s): Wang L, Heidt DG, Lee CJ, Yang H, Logsdon CD, Zhang L, Fearon ER, Ljungman M, Simeone DM, Oncogenic function of ATDC in pancreatic cancer through Wnt pathway activation and beta-catenin stabilization. Cancer cell 2009 Mar 15 (3): 207-19.PMID: 19249679