Goat anti-Aconitase 2 (aa541-555) Antibody

Pep ELISA, WB, IHC

Goat anti-Aconitase 2 (aa541-555) Antibody
Principle Name: Aconitase 2 (aa541-555)
Official Symbol: ACO2
All Name and Symbols: ACO2; aconitase 2, mitochondrial; ACONM; MGC20605; MGC33908;
aconitate hydratase; citrate hydro-lyase
Accession Number(s): NP_001089.1
Peptide Sequence: QDTYQHPPKDSSGQH, is from internal region
Human Gene ID(s): 50
Non-Human GeneID(s): 11429 (mouse); 79250 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03ug/ml.
Immunohistochemistry: In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5ug/ml.
Reference(s): Bota DA, Davies KJ. Lon protease preferentially degrades oxidized
mitochondrial aconitase by an ATP-stimulated mechanism. Nat Cell Biol. 2002 Sep; 4(9):674-80.PMID: 12198491