Goat anti-POU3F2 / BRN2 / OCT7 Antibody

Goat anti-POU3F2 / BRN2 / OCT7 Antibody
Principle Name: POU3F2 / BRN2 / OCT7
Official Symbol: POU3F2
All Name and Symbols: POU3F2; POU class 3 homeobox 2; BRN2; OCT7; OTF7; POUF3; POU domain, class 3, transcription factor 2
Accession Number(s): NP_005595.2
Peptide Sequence: AQSLVQGDYGALQSN, is from internal region (near N Terminus)
Human Gene ID(s): 5454
Non-Human GeneID(s): 18992 (mouse); 29588 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WBPeptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Cabos-Siguier B, Steunou AL, Joseph G, Alazard R, Ducoux-Petit M, Nieto L, Monsarrat B, Erard M, Clottes E, Expression and purification of human full-length N Oct-3, a transcription factor involved in melanoma growth. Protein expression and purification 2009 Mar 64 (1): 39-46.PMID: 18996486