Goat anti-SRGAP2 Antibody

Goat anti-SRGAP2 Antibody
Principle Name: SRGAP2
Official Symbol: SRGAP2
All Name and Symbols: SRGAP2; SLIT-ROBO Rho GTPase activating protein 2; FNBP2; KIAA0456; srGAP3; formin binding protein 2
Accession Number(s): NP_056141.2
Peptide Sequence: KQEDRQTPRSPDST, is from internal region
Human Gene ID(s): 23380
Non-Human GeneID(s): 14270 (mouse); 360840 (rat);
Important Comments: This antibody is expected to recognize isoform a (NP_056141.2).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IF
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentrat
Reference(s): Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T, Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization. Current biology : CB 2004 Aug 14 (16): 1436-50.PMID: 15324660