Goat anti-BIRC6 / Apollon (aa 4724 -4735) Antibody

Goat anti-BIRC6 / Apollon (aa 4724 -4735) Antibody
Principle Name: BIRC6 / Apollon (aa 4724 -4735)
Official Symbol: BIRC6
All Name and Symbols: BIRC6; baculoviral IAP repeat-containing 6; APOLLON; BRUCE; FLJ13726; FLJ13786; KIAA1289; ubiquitin-conjugating BIR-domain enzyme apollon
Accession Number(s): NP_057336.3
Peptide Sequence: SREYDGNIRQAT, is from internal region
Human Gene ID(s): 57448
Non-Human GeneID(s): 12211 (mouse); 313876 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Preliminary experiments gave bands at approx 90kDa and 70kDa in lysates of cell lines A431 and HeLa after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given
Reference(s): Lopergolo A, Pennati M, Gandellini P, Orlotti NI, Poma P, Daidone MG, Folini M, Zaffaroni N, Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. British journal of cancer 2009 Mar 100 (5): 739-46.PMID: 19223905