Goat anti-Delta-Sarcoglycan (aa245-257) Antibody

Pep ELISA, WB, IHC

Goat anti-Delta-Sarcoglycan (aa245-257) Antibody
Principle Name: Delta-Sarcoglycan (aa245-257)
Official Symbol: SGCD
All Name and Symbols: SGCD; sarcoglycan, delta (35kDa dystrophin-associated glycoprotein); 35DAG; CMD1L; DAGD; MGC22567; SG-delta; SGCDP; SGD; 35 kDa dystrophin-associated glycoprotein; 35kD dystrophin-associated glycoprotein; delta-SG; delta-sarcoglycan; dystrophin associated
Accession Number(s): NP_000328.2; NP_758447.1; NP_001121681.1
Peptide Sequence: RLPHGSYTPTGTR, is from internal region
Human Gene ID(s): 6444
Non-Human GeneID(s): 24052 (mouse); 497892 (rat);
Important Comments: This antibody is expected to recognize isoform 1 (NP_000328.2) and isoform 3 (NP_001121681.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 37kDa band observed in Human and Rat Heart lysates (calculated MW of 32.2kDa according to NP_000328.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3ug/ml.
Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Recommended concentration, 3-5ug/ml.
Reference(s): Sharma P, Tran T, Stelmack GL, McNeill K, Gosens R, Mutawe MM, Unruh H, Gerthoffer WT, Halayko AJ, Expression of the dystrophin-glycoprotein complex is a marker for human airway smooth muscle phenotype maturation. American journal of physiology. Lung cellular and molecular physiology 2008 Jan 294 (1): L57-68.PMID: 17993586