Goat anti-Orexin Receptor 2 (aa405-419) Antibody

Pep ELISA, WB

Goat anti-Orexin Receptor 2 (aa405-419) Antibody
Principle Name: Orexin Receptor 2 (aa405-419)
Official Symbol: HCRTR2
All Name and Symbols: HCRTR2; hypocretin (orexin) receptor 2; OX2R; hypocretin receptor type 2; orexin receptor type 2; ox-2-R; ox2-R
Accession Number(s): NP_001517.2
Peptide Sequence: KSLTTQISNFDNISK, is from internal region (near C Terminus)
Human Gene ID(s): 3062
Non-Human GeneID(s): 387285 (mouse); 25605 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 50kDa band observed in Mouse fetal Brain lysates (calculated MW of 52.5kDa according to Mouse NP_945200.1). Recommended concentration: 0.5-2ug/ml.
Reference(s): Chen J, Randeva HS, Genomic organization and regulation of the human orexin (hypocretin) receptor 2 gene: identification of alternative promoters. The Biochemical journal 2010 May 427 (3): 377-90.PMID: 20156195