Goat anti-CD127 / IL7R (mouse) Antibody

Pep ELISA, WB

Goat anti-CD127 / IL7R (mouse) Antibody
Principle Name: CD127 / IL7R (mouse)
Official Symbol: IL7R
All Name and Symbols: IL7R; interleukin 7 receptor; CD127; IL-7R-alpha; IL7RA; ILRA; CD127 antigen; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7RA; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin-7 receptor subunit alpha; MGC10755
Accession Number(s): NP_032398.3
Peptide Sequence: DDHSFWCHSQLE, is from internal region (near N Terminus)
Non-Human GeneID(s): 16197 (mouse); 294797 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 50kDa band observed in lysates of cell line NIH3T3 (calculated MW of 51.6kDa according to NP_032398.3). Recommended concentration: 2-4ug/ml.
Reference(s): Rajasuriar R, Booth D, Solomon A, Chua K, Spelman T, Gouillou M, Schlub TE, Davenport M, Crowe S, Elliott J, Hoy J, Fairley C, Stewart G, Cameron P, Lewin SR. Biological determinants of immune reconstitution in HIV-infected patients receiving antiretroviral therapy: the role of interleukin 7 and interleukin 7 receptor a and microbial translocation. J Infect Dis. 2010 Oct 15; 202(8):1254-64.PMID: 20812848